HelixCript™ One-Step RT-PCR Kit [Hot-Taq] is designed for sensitive amplification of the target gene in one-tube reaction from total transcripts. A reverse transcriptase, HelixCript™ Thermo Reverse Transcriptase, and HelixAmp™ Hot-Taq Polymerase are supplied as an enzyme mixture.

 

 

Figure 1. Multiplex one-step RT-PCR using HelixCript™ One-Step RT-PCR Kit [Hot-Taq].

Multiplex one-step RT-PCR in a single-tube reaction was performed with total RNA extracted from a hot pepper plant (Capsicum annuum) and then adding the indicated gene-specific primer sets. Each lane from 1 to 4 represents the progressive number of primer sets. The targets of the used primer sets are; CaAPX1 (374 bp), CaActin (461 bp), CaPF1 (615 bp), and Cadhn (761 bp). 

 

 

 

 

 

Figure 2. Viral RNA detection using HelixCript™ One-Step RT-PCR Kit [Hot-Taq].

Total RNA was isolated from a nasopharyngeal specimen of a new influenza virus-infected patient. Each reaction was performed with one of these target-specific primer sets: influenza A (InfA, 106 bp), Swine influenza A (SW InfA, 195 bp), Swine Influenza H1 (SW H1,116 bp), or RNase P (human internal control, 65 bp).

Storage

• -20 ± 5 ℃

 

Shelf life

• 12 months